Academy of Medical Sciences of I.R. Iran Archives of Iranian Medicine 1029-2977 24 9 2021 09 01 Epidemiological Study of Various HPV Strains in Cervical Fluid Samples in South-Eastern Iran, 2018–2020 678 683 10.34172/aim.2021.97 EN Reza Bahramabadi https://orcid.org/0000-0003-1920-2529 Zahra Honarvar Maryam Iranpour Mohammad Kazemi Arababadi Tania Dehesh Bahram Dabiri Abbas Mortezaeizadeh Anari Sahar Amirpour Rostami Mohammad Salajegheh Shahriar Dabiri https://orcid.org/0000-0002-5922-3976 Journal Article 10.34172/aim.2021.97 2020 11 07 2021 01 13 Background: Uterine cervical malignancy is one of the commonly detected malignancies related to the human papillomavirus (HPV) and is increasing incidentally in developing countries. Therefore, the use of an efficient diagnostic method is required as an effectual step for cervical cancer prevention and treatment. The purpose of the study was to diagnose various types of HPV in the cervical cytology specimens in the South-East of Iran. Methods: This cross-sectional study was performed on 1079 cervical fluid cytology specimens referred for two years, between 2018-2020. Polymerase chain reaction (PCR) and hybridization (INNO-LiPA HPV Genotyping EXTRA II assay) were used to determine HPV DNA and their genotypes, respectively. Results: HPV was positive in 37.7% (407 of 1079) patients with a mean age of 34.62 ± 8.82. Among positive cases, 252 (62%) had only one HPV genotype and 155 (38.05%) had multiplex HPV genotypes, which included 94 (60.7%), 38 (24.6%), 18 (11.6%) and 5 (3.2%) cases with two, three, four and five or more genotypes, respectively. The samples with multiple strains revealed 31 HPV genotypes with the four most prevalent being HPV6 (14.7%), HPV16 (10.9%), HPV53 (9.6%) and HPV51 (5.9%). Conclusion: HPV infection is the main health challenge for women that requires improved health service programs and appropriate epidemic vaccination.